human nrf2 antibody Search Results


nrf2  (R&D Systems)
92
R&D Systems nrf2
(A) Subcellular localization of <t>Nrf2</t> in CD4 + T-cells infected with HIV-1 or mock infected (3 dpi) as analyzed by biochemical fractionation. (B) Nuclear localization (left) and content (right) of Nrf2 in HIV-1 RNA + and HIV-1 RNA - cells (7 dpi) as measured by combining IF and HIV-1 RNA FISH. Scale bar = 2μm. n= number of cells from 3 donors. (C, D) Time course of the relative (infected vs mock infected) mRNA (C) and protein (D) levels of main targets of Nrf2 during the transition from productive (3-9 dpi) to latent (14 dpi) infection as measured by qPCR and western blot, respectively. TrxR1 and HMOX-1 were probed upon membrane stripping. (E) Comparison of the effect of different HIV-1 mutations on the mean relative (infected vs mock infected) mRNA level of the Nrf2 targets described in panel D. Values shown in panel B were calculated as nuclear corrected total cell fluorescence (as in) and analyzed by two-tailed unpaired t -test. For panels C, E, raw data were first normalized using GAPDH as housekeeping control and then expressed as Log 2 fold mRNA expression in infected vs mock infected cells (calculated using the 2-ΔΔCTmethod . In panel E the average of the six genes listed in Panel C is shown. For both panels data were analyzed by-two way ANOVA followed by Turkey’s post-test for multiple comparisons. ** P <0.01; *** P <0.001; **** P <0.001. Trx= thioredoxin; NQO1= NAD(P)H Quinone Dehydrogenase 1; HMOX-1= Heme Oxygenase 1; G6PD= glucose-6-phosphate dehydrogenase; GCLC= Glutamate—cysteine ligase; TrxR1= thioredoxin reductase 1.
Nrf2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
nrf2 - by Bioz Stars, 2026-06
92/100 stars
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c terminal nrf2 antibody  (R&D Systems)
93
R&D Systems c terminal nrf2 antibody
Fig. 2. Keap1 protein decreases in differentiated adipocytes, while steady-state mRNA expression for <t>Nrf2</t> and Keap1 increases. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 4, 8, 11, and 14. (A) Nrf2 and (C) Keap1 (70 kDa) protein expression was examined by immunoblotting. Black bars, full-length (FL) Nrf2 (56 kDa); gray bars, degraded (Deg) Nrf2 (40 kDa); white bars, total (Tot) Nrf2; the sums of full-length Nrf2 and degraded Nrf2 were determined to obtain relative total Nrf2 protein expression. (B) Relative steady-state Nrf2 and (D) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.
C Terminal Nrf2 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/c terminal nrf2 antibody/product/R&D Systems
Average 93 stars, based on 1 article reviews
c terminal nrf2 antibody - by Bioz Stars, 2026-06
93/100 stars
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goat polyclonal  (R&D Systems)
93
R&D Systems goat polyclonal
Fig. 2. Keap1 protein decreases in differentiated adipocytes, while steady-state mRNA expression for <t>Nrf2</t> and Keap1 increases. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 4, 8, 11, and 14. (A) Nrf2 and (C) Keap1 (70 kDa) protein expression was examined by immunoblotting. Black bars, full-length (FL) Nrf2 (56 kDa); gray bars, degraded (Deg) Nrf2 (40 kDa); white bars, total (Tot) Nrf2; the sums of full-length Nrf2 and degraded Nrf2 were determined to obtain relative total Nrf2 protein expression. (B) Relative steady-state Nrf2 and (D) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.
Goat Polyclonal, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat polyclonal/product/R&D Systems
Average 93 stars, based on 1 article reviews
goat polyclonal - by Bioz Stars, 2026-06
93/100 stars
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human mouse rat nrf2 antibody  (R&D Systems)
N/A
The Human Mouse Rat Nrf2 Antibody from R D Systems is a mouse monoclonal antibody to Nrf2 This antibody reacts with human mouse rat The Human Mouse Rat Nrf2 Antibody has been validated for the
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human nrf2 antibody  (R&D Systems)
N/A
The Human Nrf2 Antibody from R D Systems is a goat polyclonal antibody to Nrf2 This antibody reacts with human The Human Nrf2 Antibody has been validated for the following applications Western Blot
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Image Search Results


(A) Subcellular localization of Nrf2 in CD4 + T-cells infected with HIV-1 or mock infected (3 dpi) as analyzed by biochemical fractionation. (B) Nuclear localization (left) and content (right) of Nrf2 in HIV-1 RNA + and HIV-1 RNA - cells (7 dpi) as measured by combining IF and HIV-1 RNA FISH. Scale bar = 2μm. n= number of cells from 3 donors. (C, D) Time course of the relative (infected vs mock infected) mRNA (C) and protein (D) levels of main targets of Nrf2 during the transition from productive (3-9 dpi) to latent (14 dpi) infection as measured by qPCR and western blot, respectively. TrxR1 and HMOX-1 were probed upon membrane stripping. (E) Comparison of the effect of different HIV-1 mutations on the mean relative (infected vs mock infected) mRNA level of the Nrf2 targets described in panel D. Values shown in panel B were calculated as nuclear corrected total cell fluorescence (as in) and analyzed by two-tailed unpaired t -test. For panels C, E, raw data were first normalized using GAPDH as housekeeping control and then expressed as Log 2 fold mRNA expression in infected vs mock infected cells (calculated using the 2-ΔΔCTmethod . In panel E the average of the six genes listed in Panel C is shown. For both panels data were analyzed by-two way ANOVA followed by Turkey’s post-test for multiple comparisons. ** P <0.01; *** P <0.001; **** P <0.001. Trx= thioredoxin; NQO1= NAD(P)H Quinone Dehydrogenase 1; HMOX-1= Heme Oxygenase 1; G6PD= glucose-6-phosphate dehydrogenase; GCLC= Glutamate—cysteine ligase; TrxR1= thioredoxin reductase 1.

Journal: bioRxiv

Article Title: Alterations of redox and iron metabolism accompany development of HIV latency

doi: 10.1101/549014

Figure Lengend Snippet: (A) Subcellular localization of Nrf2 in CD4 + T-cells infected with HIV-1 or mock infected (3 dpi) as analyzed by biochemical fractionation. (B) Nuclear localization (left) and content (right) of Nrf2 in HIV-1 RNA + and HIV-1 RNA - cells (7 dpi) as measured by combining IF and HIV-1 RNA FISH. Scale bar = 2μm. n= number of cells from 3 donors. (C, D) Time course of the relative (infected vs mock infected) mRNA (C) and protein (D) levels of main targets of Nrf2 during the transition from productive (3-9 dpi) to latent (14 dpi) infection as measured by qPCR and western blot, respectively. TrxR1 and HMOX-1 were probed upon membrane stripping. (E) Comparison of the effect of different HIV-1 mutations on the mean relative (infected vs mock infected) mRNA level of the Nrf2 targets described in panel D. Values shown in panel B were calculated as nuclear corrected total cell fluorescence (as in) and analyzed by two-tailed unpaired t -test. For panels C, E, raw data were first normalized using GAPDH as housekeeping control and then expressed as Log 2 fold mRNA expression in infected vs mock infected cells (calculated using the 2-ΔΔCTmethod . In panel E the average of the six genes listed in Panel C is shown. For both panels data were analyzed by-two way ANOVA followed by Turkey’s post-test for multiple comparisons. ** P <0.01; *** P <0.001; **** P <0.001. Trx= thioredoxin; NQO1= NAD(P)H Quinone Dehydrogenase 1; HMOX-1= Heme Oxygenase 1; G6PD= glucose-6-phosphate dehydrogenase; GCLC= Glutamate—cysteine ligase; TrxR1= thioredoxin reductase 1.

Article Snippet: IF for Nrf2 (mab3925, 1:250; R&D systems, Minneapolis, MN) or PML (sc/966x, 1:500; Santa Cruz Biotechnology, Dallas, TX, USA) for 1-2 hr at RT.

Techniques: Infection, Fractionation, Western Blot, Membrane, Stripping Membranes, Comparison, Fluorescence, Two Tailed Test, Control, Expressing

Fig. 2. Keap1 protein decreases in differentiated adipocytes, while steady-state mRNA expression for Nrf2 and Keap1 increases. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 4, 8, 11, and 14. (A) Nrf2 and (C) Keap1 (70 kDa) protein expression was examined by immunoblotting. Black bars, full-length (FL) Nrf2 (56 kDa); gray bars, degraded (Deg) Nrf2 (40 kDa); white bars, total (Tot) Nrf2; the sums of full-length Nrf2 and degraded Nrf2 were determined to obtain relative total Nrf2 protein expression. (B) Relative steady-state Nrf2 and (D) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 2. Keap1 protein decreases in differentiated adipocytes, while steady-state mRNA expression for Nrf2 and Keap1 increases. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 4, 8, 11, and 14. (A) Nrf2 and (C) Keap1 (70 kDa) protein expression was examined by immunoblotting. Black bars, full-length (FL) Nrf2 (56 kDa); gray bars, degraded (Deg) Nrf2 (40 kDa); white bars, total (Tot) Nrf2; the sums of full-length Nrf2 and degraded Nrf2 were determined to obtain relative total Nrf2 protein expression. (B) Relative steady-state Nrf2 and (D) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Expressing, Western Blot

Fig. 3. NQO1 protein increases during limited clonal expansion and postmitotic growth arrest of differentiation. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 1, 2, 3, and 4. (A) NQO1 and (D) Keap1 protein expressions were examined by immunoblotting. (B) Relative NQO1, (C) Nrf2, and (E) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 3. NQO1 protein increases during limited clonal expansion and postmitotic growth arrest of differentiation. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 0, 1, 2, 3, and 4. (A) NQO1 and (D) Keap1 protein expressions were examined by immunoblotting. (B) Relative NQO1, (C) Nrf2, and (E) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 0, bars indicate Pr0.05 between samples. Data are expressed as mean7SD; n¼3–4.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Western Blot

Fig. 4. NQO1 and Keap1 proteins, but not mRNA, decreases as adipocyte differentiation progresses. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 4, 5, 6, 7, and 8. (A) NQO1 and (D) Keap1 protein expressions were examined by immunoblotting. (B) Relative NQO1, (C) Nrf2, and (E) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 4. Data are expressed as mean7SD; n¼3.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 4. NQO1 and Keap1 proteins, but not mRNA, decreases as adipocyte differentiation progresses. 3T3-L1 cells were differentiated as described under Materials and methods and harvested on Days 4, 5, 6, 7, and 8. (A) NQO1 and (D) Keap1 protein expressions were examined by immunoblotting. (B) Relative NQO1, (C) Nrf2, and (E) Keap1 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s for protein and one-way ANOVA Bonferroni’s for mRNA; * Pr0.05 as compared to Day 4. Data are expressed as mean7SD; n¼3.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Western Blot

Fig. 6. LiCl treatment increases NQO1 protein, but not NQO1 mRNA. 3T3-L1 cells were differentiated as described under Materials and methods, and treated with varying concentrations of LiCl at Day 8 and harvested on Day 11. A 40 mM NaCl was used as a vehicle control (VC). (A) NQO1 was examined by immunoblotting. (B) Relative NQO1 and (C) Nrf2 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s; * Pr0.05 as compared to VC for protein and mRNA. Data are expressed as mean7SD; n¼3.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 6. LiCl treatment increases NQO1 protein, but not NQO1 mRNA. 3T3-L1 cells were differentiated as described under Materials and methods, and treated with varying concentrations of LiCl at Day 8 and harvested on Day 11. A 40 mM NaCl was used as a vehicle control (VC). (A) NQO1 was examined by immunoblotting. (B) Relative NQO1 and (C) Nrf2 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s; * Pr0.05 as compared to VC for protein and mRNA. Data are expressed as mean7SD; n¼3.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Control, Western Blot

Fig. 7. Sulforaphane increases NQO1 protein and mRNA levels. 3T3-L1 cells were differentiated as described under Materials and methods, and treated with varying concentrations of sulforaphane (SFN). A 0.3% DMSO was used as a VC. (A) NQO1 was examined by immunoblotting. (B) Relative NQO1 and (C) Nrf2 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s; * Pr0.05 as compared to VC for protein and mRNA. Data are expressed as mean7SD; n¼3.

Journal: Free radical biology & medicine

Article Title: NAD(P)H:quinone oxidoreductase 1 activity reduces hypertrophy in 3T3-L1 adipocytes.

doi: 10.1016/j.freeradbiomed.2012.05.047

Figure Lengend Snippet: Fig. 7. Sulforaphane increases NQO1 protein and mRNA levels. 3T3-L1 cells were differentiated as described under Materials and methods, and treated with varying concentrations of sulforaphane (SFN). A 0.3% DMSO was used as a VC. (A) NQO1 was examined by immunoblotting. (B) Relative NQO1 and (C) Nrf2 mRNA levels were measured by SYBRGreen qPCR and normalized to 18S rRNA. Significance was determined by one-way ANOVA Dunnett’s; * Pr0.05 as compared to VC for protein and mRNA. Data are expressed as mean7SD; n¼3.

Article Snippet: We also tried the Santa Cruz N-terminal Nrf2 antibody (No. sc-13032) that gave 6–7 signals ranging from 30 to 100 kDa and another C-terminal Nrf2 antibody from R & D Systems (No. MAB3925) that gave 20þ signals ranging from 20 to 100þ kDa (data not shown).

Techniques: Western Blot